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Figure 7 | EvoDevo

Figure 7

From: POU genes are expressed during the formation of individual ganglia of the cephalopod central nervous system

Figure 7

Ino-POU4 -expression during development of I. notoides . Cartoons highlight Ino-POU4-expression as black checkered domains (see Figure 1 for color-code of ganglia). Dorsal (d)-ventral (v), anterior (a)-posterior (p), and left (l)-right (r) axes indicate the orientation. (A) Stage 19 individuals exhibit Ino-POU4-expression in the palliovisceral (pvg) and optic ganglia (og) close to the eyes (e) (dorsoposterior view). In this specimen, Ino-POU4 staining is weaker on the left side (arrowhead) than on the right side. (B) During stage 19 Ino-POU4 is expressed in the palliovisceral and optic ganglia (dorsoposterior view). (C) Stage 20 individuals exhibit Ino-POU4-expression in the optic and palliovisceral ganglia (dorsoposterior view). Note the absence of Ino-POU4 expression in the cerebral ganglia (arrowhead). (D) The optic, palliovisceral, and intrabrachial ganglia (brg) of stage 20 individuals express Ino-POU4. (E) Stage 21 individuals exhibit Ino-POU4 expression in the palliovisceral and optic ganglia (left inset), the intrabrachial and the pedal ganglia (arrowhead) (right inset). (F) Stage 22 individuals exhibit strong Ino-POU4-expression in the optic ganglia as well as in the palliovisceral and pedal ganglia (pg). No Ino-POU4 is expressed in the intrabrachial ganglia of the arms (a). (G) The Ino-POU4-expression pattern of stage 23 individuals resembles that of stage 22 individuals. (H) Vibratome section along the midline of the cephalic region during stage 25. Note the lack of Ino-POU4-expression in the vertical lobe (arrowhead). Besides the anterior (asm), middle (msm), and posterior subesophageal (psm) and supraesophageal mass, also the ventral magnocellular lobes (arrow) express Ino-POU4. (I) Stage 27 individuals exhibit only faint Ino-POU4-expression in the posterior basal lobes (arrowhead) and expression patterns of subsequent developmental stages resembles the one of stage 27 specimens. Abbreviations: bm, buccal mass; f, funnel; iy, internal yolk; m, mantle; y, yolk. The statocysts are marked by asterisks. Scale bars: A-G: 150 μm, H-I: 200 μm.

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